Review



anti αsma monoclonal antibody  (Bioss)


Bioz Verified Symbol Bioss is a verified supplier
Bioz Manufacturer Symbol Bioss manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Bioss anti αsma monoclonal antibody
    Anti αsma Monoclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acta2+(3f9)+monoclonal+antibody/ACTA2+(3F9)+Monoclonal+Antibody/pm42111644-114-10-16
    Average 94 stars, based on 25 article reviews
    anti αsma monoclonal antibody - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness
    Article Snippet: .. After washing, the slides were incubated overnight at 4 °C with primary antibodies against C/EBPβ (Santa Cruz Biotechnology, Texas, USA; sc-7962), CD68 (Proteintech Group, Hubei, China; 28058-1-AP), or ACTA2 (Bioss, Beijing, China; ab124964). .. The following day, the slides were incubated with Cy3-conjugated goat anti-rabbit IgG or Alexa Fluor 488-labeled goat anti-mouse IgG (Beyotime) for 1 h at room temperature.

    Article Title: Triamcinolone acetonide-loaded chitosan-polyethylene glycol hydrogel for preventing esophageal stricture post-endoscopic submucosal dissection
    Article Snippet: .. Afterwards, the slices were incubated with IL-1β (Cat No. AF7209, Beyotime), TNF-α (Cat No. bs-2150R, Bioss), TNF-β (Cat No. bs-0093R, Bioss), Cytokeratin 5 (Cat No. bs-1060R, Bioss), CD31 (Cat No. bs-0195R, Bioss), Collagen I (Cat No. bs-0578R, Bioss), α-SMA (Cat No. bsm-33187M, Bioss) overnight at 4°C, respectively. .. On the second day, the slices were washed and incubated with HRP-conjugated Goat anti-Rabbit IgG (H + L) (Cat No. PV-6000D, zsbio) at 37°C for 1 h, followed by staining with DAB staining solution (Cat No. PV-6000D, zsbio).

    Article Title: Gandouling protects against hepatic fibrosis in Wilson disease through the lncRNA-SNHG7/miR-29b/DNMT3A pathway.
    Article Snippet: Proteins were diluted 1:4 in 5X SDS-PAGE sample buffer (Solarbio, Item No. G8200), followed by boiling in a water bath for 15 min. After cooling to room temperature, the samples were separated on SDS-PAGE, transferred to PVDF membranes (Millipore, Item No.IPVH00010), and blocked with 5% skim milk powder. .. The membranes were then incubated overnight with antibodies against DNMT3A (Bioss, Item No. bs-23029R, 1:2000, Rabbit, 1:1000, Rabbit), p-DNMT3A (Bioss, Item No. bs-14399R, 1:2000, Rabbit), α-SMA (Bioss, Item No. Bsm-33187 M, 1:2000, Mouse; No. bs-0189R, 1:1000, Rabbit), Collagen I (Bioss, Item No. bs-7158R, 1:1000, Rabbit; No. AB260043, 1:1000, Rabbit), Beclin-1 (Abcam, Item No. ab210498, 1:2000, Rabbit; No. ab62472, 1:1000, Rabbit), LC3B (CST, Item No. 3868s, 1:1000, Rabbit; No. 43566 S, 1:1000, Rabbit), and p62 (Bioss, Item No. bs-2951R, 1:1000, Rabbit) at 4°C with gentle shaking. .. HRP-labeled secondary antibodies (1:20,000) were then incubated with blots for 1.2 h, followed by three washes with PBST (10 min/wash) and development with an ECL Ultra Sensitive Luminescence Kit (Thermo, Item No. 340958).

    Article Title: C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness.
    Article Snippet: .. After washing, the slides were incubated overnight at 4 °C with primary antibodies against C/EBPβ (Santa Cruz Biotechnology, Texas, USA; sc-7962), CD68 (Proteintech Group, Hubei, China; 28058-1-AP), or ACTA2 (Bioss, Beijing, China; ab124964). .. The following day, the slides were incubated with Cy3-conjugated goat anti-rabbit IgG or Alexa Fluor 488-labeled goat anti-mouse IgG (Beyotime) for 1 h at room temperature.

    Article Title: Exosome miR-6990-5p from MMP14 high Macrophage Promotes Fibrosis Through Th17 Cell Differentiation in Hypersensitivity Pneumonia.
    Article Snippet: Th17 cells contribute to pulmonary fibrosis, but the mechanisms driving their differentiation remain unclear.. Using single-cell RNA sequencing (scRNA-seq) and a decision tree model, we identify IL17A as a key marker in mice exposed to Saccharopolyspora rectivirgula antigen (SR-Ag).. Trajectory and T cell receptor (TCR) analyses reveal IL17A+ CD4 T cells as terminally differentiated and clonally expanded.

    other:

    Article Title: An albumin-armed fibronectin fragment assembly on stents enables immune evasion, thrombosis prevention, and self-adaptive vascular cell selectivity.
    Article Snippet: The HUVECs cells on the 24-h samples were stained with anti-Vinculin antibody (Cat. No.: FNab09800, FineTest, 1/ 100 dilution in PBS), followed by Alexa Fluor 488-conjugated antibody (Cat. No.: FNSA-0055, FineTest, 1/500 dilution in PBS), phalloidin-TRITC (1 μg/mL in PBS) and DAPI (5 μg/mL in PBS).

    Gentle:

    Article Title: Gandouling protects against hepatic fibrosis in Wilson disease through the lncRNA-SNHG7/miR-29b/DNMT3A pathway.
    Article Snippet: Proteins were diluted 1:4 in 5X SDS-PAGE sample buffer (Solarbio, Item No. G8200), followed by boiling in a water bath for 15 min. After cooling to room temperature, the samples were separated on SDS-PAGE, transferred to PVDF membranes (Millipore, Item No.IPVH00010), and blocked with 5% skim milk powder. .. The membranes were then incubated overnight with antibodies against DNMT3A (Bioss, Item No. bs-23029R, 1:2000, Rabbit, 1:1000, Rabbit), p-DNMT3A (Bioss, Item No. bs-14399R, 1:2000, Rabbit), α-SMA (Bioss, Item No. Bsm-33187 M, 1:2000, Mouse; No. bs-0189R, 1:1000, Rabbit), Collagen I (Bioss, Item No. bs-7158R, 1:1000, Rabbit; No. AB260043, 1:1000, Rabbit), Beclin-1 (Abcam, Item No. ab210498, 1:2000, Rabbit; No. ab62472, 1:1000, Rabbit), LC3B (CST, Item No. 3868s, 1:1000, Rabbit; No. 43566 S, 1:1000, Rabbit), and p62 (Bioss, Item No. bs-2951R, 1:1000, Rabbit) at 4°C with gentle shaking. .. HRP-labeled secondary antibodies (1:20,000) were then incubated with blots for 1.2 h, followed by three washes with PBST (10 min/wash) and development with an ECL Ultra Sensitive Luminescence Kit (Thermo, Item No. 340958).



    Similar Products

    94
    Bioss anti αsma monoclonal antibody
    Anti αsma Monoclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acta2+(3f9)+monoclonal+antibody/ACTA2+(3F9)+Monoclonal+Antibody/pm42111644-114-10-16
    Average 94 stars, based on 1 article reviews
    anti αsma monoclonal antibody - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Bioss α sma
    α Sma, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acta2+(3f9)+monoclonal+antibody/ACTA2+(3F9)+Monoclonal+Antibody/pm41929572-91-25-26
    Average 94 stars, based on 1 article reviews
    α sma - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Bioss mouse anti acta2
    Mouse Anti Acta2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acta2+(3f9)+monoclonal+antibody/ACTA2+(3F9)+Monoclonal+Antibody/pm41054013-123-46-50
    Average 94 stars, based on 1 article reviews
    mouse anti acta2 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    acta2  (Bioss)
    94
    Bioss acta2
    Knockdown of C/EBPβ expression inhibited MBD-Chol-induced transition of contractile VSMCs into a macrophage-like phenotype. a Modified Russell-Movat Pentachrome stain was used to show murine aortic arch tissue composition. Black for elastin fibers, yellow for collagen, blue/cyan for proteoglycan, red for collagen fibers, and black/purple for nuclei, scale bar = 100 μm. b Immunofluorescence staining was used to assess protein expression and localization in the murine aortas, blue (DAPI) for nuclei, green for C/EPBβ, and red for CD68 (representative images). The scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. c Immunofluorescence staining was used to assess protein expression and localization in VSMCs, blue for nuclei, green for <t>ACTA2,</t> and red for CD68 (representative images), scale bar = 50 μm. d Immunofluorescence staining was used to assess protein localization and expression in VSMCs, blue for nuclei, green for C/EBPβ, and red for CD68 (representative images), scale bar = 50 μm. e mRNA levels of contractile phenotype-associated markers (ACTA2, CNN1, MYH11) in VSMCs, N = 3. f mRNA levels of macrophage-like phenotype-associated markers (CD68, LGALS3) and C/EBPβ in VSMCs, N = 3. g Protein expression levels detected by WB in VSMCs (representative images). h Statistical results of protein expression levels, N = 3. * p < 0.05 and *** p < 0.001. NC= scrambled siRNA group, si-C/EBPβ = C/EBPβ siRNA group. Segment of aortic arch was obtained for staining
    Acta2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acta2+(3f9)+monoclonal+antibody/ACTA2+(3F9)+Monoclonal+Antibody+%2C+RBITC+Conjugated/pmc11962150-271-28-29
    Average 94 stars, based on 1 article reviews
    acta2 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Bioss antibody against alpha sma α sma
    Knockdown of C/EBPβ expression inhibited MBD-Chol-induced transition of contractile VSMCs into a macrophage-like phenotype. a Modified Russell-Movat Pentachrome stain was used to show murine aortic arch tissue composition. Black for elastin fibers, yellow for collagen, blue/cyan for proteoglycan, red for collagen fibers, and black/purple for nuclei, scale bar = 100 μm. b Immunofluorescence staining was used to assess protein expression and localization in the murine aortas, blue (DAPI) for nuclei, green for C/EPBβ, and red for CD68 (representative images). The scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. c Immunofluorescence staining was used to assess protein expression and localization in VSMCs, blue for nuclei, green for <t>ACTA2,</t> and red for CD68 (representative images), scale bar = 50 μm. d Immunofluorescence staining was used to assess protein localization and expression in VSMCs, blue for nuclei, green for C/EBPβ, and red for CD68 (representative images), scale bar = 50 μm. e mRNA levels of contractile phenotype-associated markers (ACTA2, CNN1, MYH11) in VSMCs, N = 3. f mRNA levels of macrophage-like phenotype-associated markers (CD68, LGALS3) and C/EBPβ in VSMCs, N = 3. g Protein expression levels detected by WB in VSMCs (representative images). h Statistical results of protein expression levels, N = 3. * p < 0.05 and *** p < 0.001. NC= scrambled siRNA group, si-C/EBPβ = C/EBPβ siRNA group. Segment of aortic arch was obtained for staining
    Antibody Against Alpha Sma α Sma, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acta2+(3f9)+monoclonal+antibody/ACTA2+(3F9)+Monoclonal+Antibody/10__1620_slash_tjem__2025__j040-61-3-9
    Average 94 stars, based on 1 article reviews
    antibody against alpha sma α sma - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Bioss anti α sma
    Knockdown of C/EBPβ expression inhibited MBD-Chol-induced transition of contractile VSMCs into a macrophage-like phenotype. a Modified Russell-Movat Pentachrome stain was used to show murine aortic arch tissue composition. Black for elastin fibers, yellow for collagen, blue/cyan for proteoglycan, red for collagen fibers, and black/purple for nuclei, scale bar = 100 μm. b Immunofluorescence staining was used to assess protein expression and localization in the murine aortas, blue (DAPI) for nuclei, green for C/EPBβ, and red for CD68 (representative images). The scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. c Immunofluorescence staining was used to assess protein expression and localization in VSMCs, blue for nuclei, green for <t>ACTA2,</t> and red for CD68 (representative images), scale bar = 50 μm. d Immunofluorescence staining was used to assess protein localization and expression in VSMCs, blue for nuclei, green for C/EBPβ, and red for CD68 (representative images), scale bar = 50 μm. e mRNA levels of contractile phenotype-associated markers (ACTA2, CNN1, MYH11) in VSMCs, N = 3. f mRNA levels of macrophage-like phenotype-associated markers (CD68, LGALS3) and C/EBPβ in VSMCs, N = 3. g Protein expression levels detected by WB in VSMCs (representative images). h Statistical results of protein expression levels, N = 3. * p < 0.05 and *** p < 0.001. NC= scrambled siRNA group, si-C/EBPβ = C/EBPβ siRNA group. Segment of aortic arch was obtained for staining
    Anti α Sma, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acta2+(3f9)+monoclonal+antibody/ACTA2+(3F9)+Monoclonal+Antibody/10__1620_slash_tjem__2025__j040-72-19-22
    Average 94 stars, based on 1 article reviews
    anti α sma - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Knockdown of C/EBPβ expression inhibited MBD-Chol-induced transition of contractile VSMCs into a macrophage-like phenotype. a Modified Russell-Movat Pentachrome stain was used to show murine aortic arch tissue composition. Black for elastin fibers, yellow for collagen, blue/cyan for proteoglycan, red for collagen fibers, and black/purple for nuclei, scale bar = 100 μm. b Immunofluorescence staining was used to assess protein expression and localization in the murine aortas, blue (DAPI) for nuclei, green for C/EPBβ, and red for CD68 (representative images). The scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. c Immunofluorescence staining was used to assess protein expression and localization in VSMCs, blue for nuclei, green for ACTA2, and red for CD68 (representative images), scale bar = 50 μm. d Immunofluorescence staining was used to assess protein localization and expression in VSMCs, blue for nuclei, green for C/EBPβ, and red for CD68 (representative images), scale bar = 50 μm. e mRNA levels of contractile phenotype-associated markers (ACTA2, CNN1, MYH11) in VSMCs, N = 3. f mRNA levels of macrophage-like phenotype-associated markers (CD68, LGALS3) and C/EBPβ in VSMCs, N = 3. g Protein expression levels detected by WB in VSMCs (representative images). h Statistical results of protein expression levels, N = 3. * p < 0.05 and *** p < 0.001. NC= scrambled siRNA group, si-C/EBPβ = C/EBPβ siRNA group. Segment of aortic arch was obtained for staining

    Journal: Signal Transduction and Targeted Therapy

    Article Title: C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness

    doi: 10.1038/s41392-025-02196-w

    Figure Lengend Snippet: Knockdown of C/EBPβ expression inhibited MBD-Chol-induced transition of contractile VSMCs into a macrophage-like phenotype. a Modified Russell-Movat Pentachrome stain was used to show murine aortic arch tissue composition. Black for elastin fibers, yellow for collagen, blue/cyan for proteoglycan, red for collagen fibers, and black/purple for nuclei, scale bar = 100 μm. b Immunofluorescence staining was used to assess protein expression and localization in the murine aortas, blue (DAPI) for nuclei, green for C/EPBβ, and red for CD68 (representative images). The scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. c Immunofluorescence staining was used to assess protein expression and localization in VSMCs, blue for nuclei, green for ACTA2, and red for CD68 (representative images), scale bar = 50 μm. d Immunofluorescence staining was used to assess protein localization and expression in VSMCs, blue for nuclei, green for C/EBPβ, and red for CD68 (representative images), scale bar = 50 μm. e mRNA levels of contractile phenotype-associated markers (ACTA2, CNN1, MYH11) in VSMCs, N = 3. f mRNA levels of macrophage-like phenotype-associated markers (CD68, LGALS3) and C/EBPβ in VSMCs, N = 3. g Protein expression levels detected by WB in VSMCs (representative images). h Statistical results of protein expression levels, N = 3. * p < 0.05 and *** p < 0.001. NC= scrambled siRNA group, si-C/EBPβ = C/EBPβ siRNA group. Segment of aortic arch was obtained for staining

    Article Snippet: After washing, the slides were incubated overnight at 4 °C with primary antibodies against C/EBPβ (Santa Cruz Biotechnology, Texas, USA; sc-7962), CD68 (Proteintech Group, Hubei, China; 28058-1-AP), or ACTA2 (Bioss, Beijing, China; ab124964).

    Techniques: Knockdown, Expressing, Modification, Staining, Immunofluorescence

    Knockout of C/EBPβ in VSMCs alleviated hyperlipidemia-induced arterial stiffness. a Statistical result of changes in aPWV, N = 6. b aortic contractile function in vivo, N = 6. c Flow cytometry results of the Control group and the HF (ApoE −/− ) group, N = 6. d Modified Russell–Movat Pentachrome stain was used to show murine arterial tissue composition. Black for elastin fibers, yellow for collagen, blue/cyan for proteoglycan, red for collagen fibers, and black/purple for nuclei (representative images), scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. e Immunofluorescence staining was used to assess protein expression and localization in the murine aortas, blue for nuclei, green for C/EBPβ, and red for CD68 (representative images), scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. f From left to right, respectively, are statistical results of vascular wall/lumen measured ex vivo, N = 6, statistical results of elastic fibers, N = 6; Statistical results of proteoglycan, N = 6, Statistical results of CD68 fluorescence area, N = 6. ACTA2 + = ACTA2-positive cells, CD68 + = CD68-positive cells, CD68 + /CEBPb + = CD68/C/EBPβ double-positive cells. Control = ApoE −/− mice fed with chow food, HF (ApoE −/− ) = ApoE −/− mice fed with a 60% high-fat diet, HF (C/EBPβ CKO /ApoE −/− ) = ApoE −/− /C/EBPβ fl/fl- -Tagln cre mice fed with a 60% high-fat diet. * p < 0.05 and *** p < 0.001. Segment of aortic arch was obtained for staining

    Journal: Signal Transduction and Targeted Therapy

    Article Title: C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness

    doi: 10.1038/s41392-025-02196-w

    Figure Lengend Snippet: Knockout of C/EBPβ in VSMCs alleviated hyperlipidemia-induced arterial stiffness. a Statistical result of changes in aPWV, N = 6. b aortic contractile function in vivo, N = 6. c Flow cytometry results of the Control group and the HF (ApoE −/− ) group, N = 6. d Modified Russell–Movat Pentachrome stain was used to show murine arterial tissue composition. Black for elastin fibers, yellow for collagen, blue/cyan for proteoglycan, red for collagen fibers, and black/purple for nuclei (representative images), scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. e Immunofluorescence staining was used to assess protein expression and localization in the murine aortas, blue for nuclei, green for C/EBPβ, and red for CD68 (representative images), scale bars correspond to 100 μm for low-magnification images and 20 μm for high-magnification views. f From left to right, respectively, are statistical results of vascular wall/lumen measured ex vivo, N = 6, statistical results of elastic fibers, N = 6; Statistical results of proteoglycan, N = 6, Statistical results of CD68 fluorescence area, N = 6. ACTA2 + = ACTA2-positive cells, CD68 + = CD68-positive cells, CD68 + /CEBPb + = CD68/C/EBPβ double-positive cells. Control = ApoE −/− mice fed with chow food, HF (ApoE −/− ) = ApoE −/− mice fed with a 60% high-fat diet, HF (C/EBPβ CKO /ApoE −/− ) = ApoE −/− /C/EBPβ fl/fl- -Tagln cre mice fed with a 60% high-fat diet. * p < 0.05 and *** p < 0.001. Segment of aortic arch was obtained for staining

    Article Snippet: After washing, the slides were incubated overnight at 4 °C with primary antibodies against C/EBPβ (Santa Cruz Biotechnology, Texas, USA; sc-7962), CD68 (Proteintech Group, Hubei, China; 28058-1-AP), or ACTA2 (Bioss, Beijing, China; ab124964).

    Techniques: Knock-Out, In Vivo, Flow Cytometry, Control, Modification, Staining, Immunofluorescence, Expressing, Ex Vivo, Fluorescence